FISH ANALYSIS PML/RARA
Marquette General Health System

General Info

Alpha Code

   CYTOGNREQ

MGH LIS Test No

    

Schedule

   Monday-Friday

Testing Time

    

Testing Lab

   Marquette General Hospital

QORR Test Code

   FISHRA

Specimen Info

Type

   Bone Marrow or Malignant Blood  

Volume

   3.0 mL Bone Marrow or 5.0 mL Blood

Temperature

   Ambient

Preservative

    

Collection Info

   Bone Marrow is the tissue of choice for 
initial diagnosis of most neoplastic 
specimens.  Malignant blood can be 
used in certain cases to monitor minimal
residual disease.

Prefer a minimum of 3.0 mL of bone marrow 
or 5.0 mL of malignant blood.  Draw into 
green-top (sodium heparin) tube(s), invert 
several times to mix (clotted specimens 
may not work and can compromise results).  
Label vial with the patient's name and a 
unique identifying number (MGHS encounter #
 or MR #).  Maximum time from 
collection should be 24 hours.

SPECIMENS CANNOT BE FROZEN
 
Specimen Acceptability

Methods
Interphase set-up and analysis. A minimum of 500 interphase nuclei will be analyzed.

Clinical Utilities
Diagnosis of PML-RARA gene fusion characteristic of the t(15;17)(q22;q21) associated with AML M3 or acute promyelocytic leukemia (APL). Treatment of AML M3 with all-trans retinoic acid (ATRA) therapy is considered an emergency and is processed as STAT.

CPT Codes
88271x2 (DNA probe, each), 88275x2 (Interphase in situ hybridization), 88291 (Interpretation and reporing). The following CPT codes are added at additional charge as needed: 88230 (Cell culture, lymphocytes).

Reference Range
0-0.6% fusion=Normal
Above 0.6% fusion = Abnormal

Component Information

Collection Notes

Additional Comments

 

Questions or Comments email support@mgh.org

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