JAK2 V617F MUTATION DETECTION
Marquette General Health System

General Info

Alpha Code

   JAK2

MGH LIS Test No

   799

Schedule

   Monday through Friday

Testing Time

   1 Day

Testing Lab

   Mayo Labs

QORR Test Code

   JAK2

Specimen Info

Type

   Blood, Bone Marrow, or Extracted DNA  

Volume

   See Below

Temperature

   See Below

Preservative

    

Collection Info

   Specimen must arrive within 72 hours of draw. 

Submit only 1 of the following specimens: 

Preferred:

Blood
Draw blood in a lavender-top (EDTA) tube(s), and send 3.0 mL of

EDTA whole blood in original VACUTAINER(S). Invert several 

times to mix blood. Forward unprocessed whole blood promptly at 

ambient temperature only. Specimen cannot be frozen.

Note:      If ordering electronically, please complete and submit a

                  "MayoConnect Additional Test Information Form" (Supply

                  T357 or see Special Instructions) with the specimen. If not

                  ordering electronically, please complete and submit a

                  "Hematopathology/Molecular Oncology/Cytogenetics

                  Request Form" (Supply T241) with the specimen.

 

Bone Marrow

Place 3.0 mL of bone marrow in a lavender-top (EDTA) tube(s)

and send in original VACUTAINER(S). Invert several times to mix

bone marrow. Forward unprocessed bone marrow promptly at 

ambient temperature only. Specimen cannot be frozen.

Note:      If ordering electronically, please complete and submit a

                  "MayoConnect Additional Test Information Form" (Supply

                  T357 or see Special Instructions) with the specimen. If not

                  ordering electronically, please complete and submit a 

                  "Hematopathology/Molecular Oncology/Cytogenetics

                  Request Form" (Supply T241) with the specimen.

 

Alternate:

Extracted DNA

DNA extracted at client site is not the preferred specimen type, 

but it will be accepted as long as it conforms to the following criteria:

1.   Accepted for blood, bone marrow, and fluid only

2.   Follow acceptable fluid requirements (no specimens 

      collected in heparin)

3.   No extracted DNA from fresh, frozen, or paraffin-embedded tissue

4.   Minimum volume of 5.0 ug

5.   Send specimen labeled with concentration and volume.

6.   Prefer shipment ambient

 

DNA will be assessed for quality. If it is deemed unacceptable, 

testing will be cancelled.

Note:      If ordering electronically, please complete and submit a

                  "MayoConnect Additional Test Information Form" (Supply

                  T357 or see Special Instructions) with the specimen. If not

                  ordering electronically, please complete and submit a 

                  "Hematopathology/Molecular Oncology/Cytogenetics

                  Request Form" (Supply T241) with the specimen.
 
Specimen Acceptability

Methods
Point Mutation Detection using Polymerase Chain Reaction (PCR)

Clinical Utilities
Aiding in the distinction between a reactive cytosis and a chronic myeloproliferative disorder.

CPT Codes
"HPGDE, DNA extraction" 83891 "JAK2 V617F Mutation Detection " 83896/Nucleic acid probe 83898/Amplification of patient nucleic acid "Interpretation and Report" 83912

Reference Range
Separate report sent.

Component Information

Collection Notes

Additional Comments

 

Questions or Comments email support@mgh.org

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