LEUKEMIA/LYMPHOMA IMMUNOPHENOTYPING BY FLOW CYTOMETRY
Marquette General Health System
HLAB/HOL Code |
FLOW | |
MGH LIS Test No |
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Schedule |
Monday - Friday | |
Testing Time |
8 Hours | |
Testing Lab |
Marquette General Hospital | |
QORR Test Code |
FLOW | |
Type |
Peripheral Blood | |
Volume |
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Temperature |
Ambient | |
Preservative |
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Collection Info |
Draw 5 to 10mL of peripheral blood in a yellow-top (ACD) or purple-top (EDTA) tube. Do not transfer blood to other containers. Send a current CBC printout and 4 unstained slides. Forward at room temperature only. SAMPLES CANNOT BE FROZEN. Submit a requisition clearly stating "Leukemia / Lymphoma Immunophenotyping" as well as the appropriate collection time and date of sample. Submit the name and telephone number of the ordering physician along with a brief diagnostic history of the patient, including the clinical differential diagnosis. |
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Type |
Bone Marrow | |
Volume |
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Temperature |
Ambient | |
Preservative |
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Collection Info |
Send 5 to 10mL of ACD bone marrow and 4 unstained slides. Forward at room temperature only. SAMPLES CANNOT BE FROZEN. Submit a requisition clearly stating "Leukemia / Lymphoma Immunophenotyping" as well as the appropriate collection time and date of sample. Submit the name and telephone number of the ordering physician along with a brief diagnostic history of the patient, including the clinical differential diagnosis. Please Send a current CBC printout with each request as well. |
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Type |
CSF | |
Volume |
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Temperature |
Ambient | |
Preservative |
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Collection Info |
The volume of fluid necessary to adequately perform immunophenotyping depends on the cellularity of the fluid. Therefore, it is required to have a cell count done on the sample prior to shipping and enclose a copy of that result. Due to the rapid degradation of cells in a sample of this type, it is essential that at least 2 unstained cytocentrifuged smears accompany the sample in order to properly correlate morphologic evaluation with phenotype data. Forward at room temperature only. SAMPLES CANNOT BE FROZEN. Submit a requisition clearly stating "Leukemia / Lymphoma Immunophenotyping" as well as the appropriate collection time and date of sample. Submit the name and telephone number of the ordering physician along with a brief diagnostic history of the patient, including the clinical differential diagnosis. Please Send a current CBC printout with each request as well. |
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Type |
Body Fluids | |
Volume |
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Temperature |
Ambient | |
Preservative |
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Collection Info |
The volume of fluid necessary to adequately perform immunophenotyping depends on the cellularity of the fluid. A volume of 20mL is usually sufficient. If possible, fluids other than CSF should be anticoagulated with heparin (1U/mL). Due to the rapid degradation of cells in a sample of this type, it is essential that at least 2 unstained cytocentrifuged smears accompany the sample in order to properly correlate morphologic evaluation with phenotype data. Forward at room temperature only. SAMPLES CANNOT BE FROZEN. Submit a requisition clearly stating "Leukemia / Lymphoma Immunophenotyping" as well as the appropriate collection time and date of sample. Submit the name and telephone number of the ordering physician along with a brief diagnostic history of the patient, including the clinical differential diagnosis. Please Send a current CBC printout with each request as well. |
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Type |
Tissue | |
Volume |
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Temperature |
Refrigerate | |
Preservative |
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Collection Info |
SPECIMEN MUST ARRIVE WITHIN 24 HOURS OF COLLECTION. Size of the tissue sample depends on cellular constituents, but usually, a 0.5cm x 0.5cm x 0.5cm portion is sufficient. Obtain sample and place directly into a screw-capped, sterile container with approximately 15mL of Hank's balanced salt solution. DO NOT MINCE. SAMPLES CANNOT BE FROZEN. Submit a requisition clearly stating "Leukemia / Lymphoma Immunophenotyping" as well as the appropriate collection time and date of sample. Submit the name and telephone number of the ordering physician along with a brief diagnostic history of the patient, including the clinical differential diagnosis. Please Send a current CBC printout with each request as well. |
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Clinical Utilities
Analysis by flow cytometry involves the selection of the appropriate panel of antibodies directed against antigens of lymphoid and/or myeloid cell lineages. A hematopathologist selects the necessary panel(s) according to criteria based on clinical information, specimen source, morphologic review and any other instructions conveyed by the referring physician. Accordingly, each case is approached with a specifically-tailored panel.
This procedure is applicable for all cases of acute lymphoblastic or myelogenous leukemias, chronic lymphoproliferative disorders and malignant lymphomas. For Leukemia / Lymphoma Immunophenotyping there is no ordering code, simply send the sample(s) and all relevant paperwork according to the instructions listed below.
Note: For the evaluation of acquired or inherited immunodeficiancies, the T-and-B Cell Quantitation by Flow Cytometry (TBSM) should be ordered.
CPT Codes
88184 for the 1st marker used (technical component)
88185 for each additional marker used (technical component)
88187 for interpretation of 2 to 8 markers (professional component)
88188 for interpretation of 9 to 15 markers (professional component)
88189 for interpretation of 16 or more markers (professional component)
Reference Range Component Information Additional Comments Questions or Comments email support@mgh.org
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