UROPORPHYRINOGEN III SYNTHASE (CO-SYNTHASE) (UPG III S), ERYTHROCYTES
Marquette General Health System

General Info

Alpha Code

   UPS

MGH LIS Test No

   695

Schedule

   Varies

Testing Time

   7 days

Testing Lab

   Mayo Labs

QORR Test Code

   UPS

Specimen Info

Type

      

Volume

    

Temperature

   Frozen

Preservative

    

Collection Info

   If washing cells at your facility, please follow these instructions.
1. Draw blood in green-top (heparin) tube(s), and process 5.0 mL of 
    heparinized whole blood as follows:
    A. Place on wet ice immediately.
    B. Transfer to a 12-mL graduated centrifuge tube.
    C. Centrifuge for 10 minutes at 2,000 rpm.
    D. Record volume of packed cells.
    E. Discard supernatant plasma.
    F. Wash erythrocytes 3 times by resuspension in 5.0 mL of cold
       0.9% saline.
    G. Resuspend packed cells to a volume of 5.0 mL with 0.9% saline.
 2. Send washed erythrocyte suspension frozen in plastic vial
    on dry ice.
NOTE:  VOLUME OF PACKED CELLS AND TOTAL VOLUME OF ERYTHROCYTE SUSPEN-
       SION (RED CELLS + SALINE) ARE REQUIRED ON REQUEST FORM FOR
       PROCESSING.    DO NOT WRITE SUSPENSION INFORMATION ONLY ON THE
       TRANSFER TUBE.  IT MUST BE INCLUDED ON THE REQUEST FORM.
 
Specimen Acceptability

Methods
Incubation of Erythrocytes and Comparison of Uroporphyrinogen I and Uroporphyrinogen III Formed Useful for diagnosis of erythropoietic uroporphyria ("congenital erythropoietic porphyria").

Clinical Utilities

CPT Codes
84311

Reference Range
> or = 75 Relative Units (normal)
< or = 10 Relative Units (erythropoietic uroporphyria)
 
See "Porphyrinogens, Porphyrins, and Porphyrias."      

Component Information

Collection Notes

Additional Comments

 

Questions or Comments email support@mgh.org

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