CUTANEOUS IMMUNOFLUORESCENCE, BIOPSY
Marquette General Health System
Alpha Code |
C8394 | |
MGH LIS Test No |
8394 | |
Schedule |
Monday through Friday | |
Testing Time |
1 day | |
Testing Lab |
Mayo Labs | |
QORR Test Code |
C8394 | |
Type |
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Volume |
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Temperature |
Media-Amb\Refr OK\No media-Frozen | |
Preservative |
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Collection Info |
Punch biopsy (4 mm) of uninvolved or involved skin as per complete instructions. Skin or mucosal specimens can be sent by using either the transport medium or the snap-frozen procedure. The practical value of using transport medium (supplied by Mayo Medical Laboratories) is recognized for direct immunofluorescence testing. However, we have found a loss in sensitivity of approximately 10% with the transport medium as compared with snap-frozen tissue. This may necessitate a repeat biopsy if the result seems to be false-negative. The assay cannot be performed on specimens fixed in formalin. TRANSPORT MEDIUM METHOD Immediately drop specimen into provided vial of transport medium. Seal tightly. SNAP-FROZEN METHOD 1. Immediately drop specimen into liquid nitrogen and allow to freeze thoroughly (do not allow specimen to desiccate). If liquid nitro- gen is not available, the specimen may be frozen by placing it on a small square of aluminum foil on a block of dry ice. The former is preferred. 2. Immediately wrap specimen carefully in aluminum foil. At no time should the specimen be allowed to thaw. Wrap as you would a party favor or a piece of taffy candy. 3. Place the wrapped specimen into the prelabeled plastic vial and seal tightly. 4. Send specimen frozen on dry ice. NOTE: 1. BIOPSY SITE, WHETHER THE BIOPSY WAS OBTAINED FROM SUN-EXPOSED VERSUS UNEXPOSED SKIN, WHETHER IT IS FROM PERILESIONAL, INVOLVED, OR UNINVOLVED SKIN, AND DATE OF COLLECTION ARE REQUIRED ON REQUEST FORM FOR PROCESSING. 2. Please complete a "Pathology/Dermatology/Cytology Request Form"and forward it with the specimen. This form is supplied by Mayo Medical Laboratories. Requisitions for this procedure cannot be processed unless the information requested is supplied. |
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Clinical Utilities CPT Codes Reference Range
88323/cutaneous immunofluorescence, biopsy
88346/x5 direct immunofluorescence staining
Report includes description and interpretation of staining patterns.
Component Information
Additional Comments Questions or Comments email support@mgh.org
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