HISTOCHEMISTRY OF SENT-IN MUSCLE SPECIMEN - MAXIMUM
Marquette General Health System
Alpha Code |
M5334 | |
MGH LIS Test No |
5334 | |
Schedule |
Varies/One to two times per week | |
Testing Time |
7 days | |
Testing Lab |
Mayo Labs | |
QORR Test Code |
M5334 | |
Type |
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Volume |
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Temperature |
Frozen | |
Preservative |
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Collection Info |
SEND SPECIMEN MONDAY, TUESDAY, OR WEDNESDAY ONLY. Obtain the biopsy from a muscle that is definitely affected, but not so severely affected that much of it is replaced by fatty or fibrous connective tissue. In addition, the involved muscle should not be one that has been traumatized by injections or by electromyographic studies. Typically, the triceps, biceps, or vastus lateralis is chosen. (We find deltoid or gastrocnemius muscles less satisfactory and more subject to artifact.) Excise a specimen approximately 1 cm by 0.5 cm with minimal trauma, by dissecting along the long axis of the muscle. Immediately after removal, the specimen must be flash frozen. Plunge the specimen into a slurry of dry ice and acetone or into a slurry of dry ice and absolute alcohol. It is important that at least 80% of the slurry should be dry ice, and the slurry must be stirred constantly to assure that a uniform temperature of -70(C) is achieved. The immersion into the freezing solution is to last not more than is needed to completely freeze the specimen, and during the period of immersion, the specimen should be swirled around in the slurry. The well frozen specimen has a white, chalky color. Prolonged immersion in the quenching mixture should be avoided, as the specimen can become permeated by alcohol or acetone. The total freezing time is usually <10 seconds. Im- mediately after this, the frozen specimen is removed and packed in dry ice, or temporarily stored at -70 degrees C. The specimen must not thaw between the time of freezing and analysis. Send specimen frozen in Tissue Specimen Bag (T303) on dry ice. Due to the nature of testing to be done, we do not recommend the use of a muscle clamp or freezing the specimen using a mounting media, i.e., OCT. NOTE: Please complete a "Muscle Histochemistry Information Sheet", INCLUDING PERTINENT STUDIES, SUCH AS CREATINE KINASE (CK) AND ELECTROMYOGRAPHIC STUDIES, THE CLINICAL DIAGNOSIS, NAME OF MUSCLE, DATE OF BIOPSY, AND BRIEF CLINICAL HISTORY on a "Pathology/Dermatopathology/Cytology Request Form" and forward it with the specimen. This form is supplied by Mayo Medical Laboratories. Requisitions for this specimen cannot be processed unless the information requested is supplied. |
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Clinical Utilities CPT Codes Reference Range
88314/Hematoxylin-and-eosin water soluble stain
88314/Trichrome water soluble stain
88314/Periodic acid-schiff stain
88314/Oil red O stain
88314/Congo red stain
88314/TB ATPase alkaline stain
88314x3/ATPase acid alkaline stain
88319/NADH dehydrogenase stain
88319/Succinic dehydrogenase stain
88319/Phosphorylase stain
88319/Acid phosphatase stain
88319/Alpha-naphthyl stain
88319/Acetate non-specific esterase stain
88319/Cytochrome oxidase stain
88305/Surgical pathology exam
The laboratory will provide an interpretive report.
Component Information
Additional Comments Questions or Comments email support@mgh.org
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